# Data

## Raw Data

The raw flow cytometry files have been stored on the CaltechDATA research data depository. It can be downloaded from the link below:

- Flow cytometry files (
`.csv`

) | DOI: 10.22002/D1.1241

We note that a subset of raw data files were unfortunately lost and only the preprocessed data is maintained. Please see the README file along with the raw data for more information.

## Processed data

The processed data is hosted on this website and can be downloaded via the following links.

*Main Text Data*

- Compiled data

Experimental fold-change measurements of mutant and wild-type strains - Summarized data

Experimental fold-change measurements summarized with the mean and standard error for each unique strain and IPTG concentration. - Empirical free energy statistics

Inferred mean fold-change, standard deviation, empirical free energy, and change in free energy. - DNA binding energy inference statistics

Summarized results for inference of the DNA binding energy for each DNA binding mutant strain. - DNA binding energy inference MCMC samples

MCMC samples from estimation of DNA binding energy for each DNA binding mutant strain. - Inducer dissociation constant inference statistics

Summarized results for inference of the inducer binding constants (Ka and Ki alone) for all inducer binding mutant strains. - Inducer dissociation constant inference MCMC samples

MCMC samples from estimation of inducer binding constants (Ka and Ki alone) for all inducer binding mutant strains. - Allosteric parameter inference statistics

Summarized results for inference of all allosteric parameters for the inducer binding mutant strains. - Allosteric parameter inference MCMC samples

MCMC samples from estimation of all allosteric parameters for inducer binding mutant strains.

*Supplementary Data*

- Prior predictive samples for DNA binding energy inference
- Simulation based calibration for DNA binding energy inference
- Prior predictive samples for empirical free energy inference
- Simulation based calibration for empirical free energy inference
- Simulated fold-change measurements for determining empirical free energy sensitivity limits
- Inference statistics of simulated fold-change data
- Inference statistics of empirical free energy from Razo-Mejia et al. 2018
- Prior predictive samples for inference of allosteric parameters
- Simulation based calibration for inference of allosteric parameters
- Global DNA binding energy inference MCMC samples
- Global DNA binding energy inference statistics
- Global allosteric parameter inference MCMC samples
- Global allosteric parameter inference statistics

*Auxiliary Data Files*