• Lab | 19:00 - 22:00 in College B.416 (Wet Lab) and College B.214 (Optical Tweezer Lab).

Handouts

Every student will have the opportunity to build an optical tweezer microscope as well as perform wet-lab experiments for the quantification of gene expression. Optional handouts for these materials can be found here:

• Quantifying Gene Expression: Titrating the Lac repressor

• PBoC textbook: For the optical tweezer experiment, page 153-154 and the Computational Exploration section on page 207-208 might be useful if you’d like to determine the trap stiffness. Image Analysis with Rapid and Accurate Two-Dimensional Gaussian Fitting by Anthony and Granick provides an advanced and generally more accurate approach to calculating the trap stiffness.