APh 162 Lab 1
Group HIG
Ian Krajbich
Hesper Rego
Jaime Valle
The resolution target and corresponding sizes of elements. Note: to get the size of a line one must invert the lines/mm.
Part II: Show and Tell
Using fluorescent proteins, we were able to look at different parts of the cell - mitochondria and actin - since each was excited and fluoresced at different wavelengths of light.
We then took different pictures of two different yeast strains.
Part I: Microscope Callibration
Since the sizes of the elements on the resolution target are known we can use these pictures to scale future images.
Objectives
In this weeks lab, we will use a resolution target to calibrate the microscope and to get a sense for the scales of actin, mitochondria, and different strains of yeast.
Conclusion
We were able to callibrate the microscope at magnifications of 10, 20, and 100. We then put scale bars on the pictures we took of mitochondria, actin , and yeast. In this way we now have a sense of scale for things at the cellular level,
