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Phillips Group Bootcamp 2005

Instructors: Rob Phillips , Hernan Garcia , Tristan Ursell
August 1st - 9th, 2005

This was our first year running an intense, hands-on physical biology lab course. Our participants were as varied as our experiments; with professors and scholars coming from Brandeis, Princeton, Brown, and Caltech. Our experiments dipped into gene regulation and transformation, bio-polymerization, membrane mechanics, size and time scales of developing organisms(urchins), and much more. See the schedule below for a rough idea!

[ Schedule | The Size of Things | The Rate of Things | DNA Science | Lipid Membrane Mechanics | Biological Polymers | Optical Tweezers | In the Future ]

Single Cell Fluorescence Analysis

Lipid Membrane Analysis

Optical Tweezer Analysis
Image Processing Tutorial Sessions

 

Bootcamp Schedule

Day 1:

The Size of Things

Using E. Coli and Yeast as the standard cellular ruler.

Exploring the sizes of other organisms: red blood cells, 3T3 cells, stentor...

The Rate of Things

Made our first attempts at sea urchin fertilization; measured the growth rates of E. coli; explored how enzyme concentration affects reaction kinetics.

Plated and transformed E. coli to express GFP in preparation for DNA Science

Day 2:

Finished up: The Rate of Things

Successfully extracted Sea Urchin gametes, watched fertilization and the first few mitotic events.

DNA Science

Setup primers and templates for PCR and qPCR reactions.

Began our Lambda genome restriction digests.

Subsequently ran gels on standard PCR reactions and restriction digest products.

Days 3-5:

Each group of 2 split up to spend an entire day working on:

Optical Tweezers

Lipid Membrane Mechanics

Biological Polymers

 
At various points during the following week, groups analyzed their data from the Optical Tweezers (Dave Wu), Lipid Mechanics (Tristan), and single cell fluoresence measurements (Hernan).


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