Biological Polymers


[ Fluorescent Microtubule Polymerization | Actin Polymerization Kinetics ]


 

Fluorescent Microtubule Polymerization

 

Fluoresenct microtubules can be polymerized at different tubulin monomer concentrations. Independently of the fact that the density of microtubules is changing, notice how he distribution of lengths is changing at the same time.

 

Custom image analysis code can be written to measure the distribution of lengths or the angle-angle correlation, which would give information about the the flexibility of the polymer.


 

Actin Polymerization Kinetics

 

Pyrene-labelled actin becomes fluorescent upon polymerization, but remains dark when it is in the monomer form. By monitoring the fluorescent signal we can measure the kinetics of polymerization.

This bulk experiment allows us to ask question about how the actin microscopical properties affect the bulk behaviour of the polymer.